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Heat‐shock tagging: a simple method for expression and isolation of plant genome DNA flanked by T‐DNA insertions
Author(s) -
Matsuhara Shio,
Jingu Fumito,
Takahashi Taku,
Komeda Yoshibumi
Publication year - 2000
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2000.00716.x
Subject(s) - genome , polyadenylation , transcription (linguistics) , biology , dna , gene , genetics , promoter , microbiology and biotechnology , gene expression , linguistics , philosophy
Summary This paper describes expression profiles of the Arabidopsis HSP18.2 heat‐shock gene promoter by using three different reporter genes, and the application of this promoter to a method we have developed to drive heat‐shock‐dependent transcription of plant genome DNA flanked by T‐DNA insertions. We show that, irrespective of the location of the T‐DNA insertion, an HSP18.2 promoter towards the left border of the T‐DNA effectively induces transcription of flanking genome sequences in response to heat shock. If polyadenylated, tagged transcripts can be easily identified by RT–PCR.