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A lipochito‐oligosaccharide, Nod factor, induces transient calcium influx in soybean suspension‐cultured cells
Author(s) -
Yokoyama Tadashi,
Kobayashi Naoki,
Kouchi Hiroshi,
Minamisawa Kiwamu,
Kaku Hisatoshi,
Tsuchiya Kenichi
Publication year - 2000
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2000.00713.x
Subject(s) - nod , cytosol , bradyrhizobium japonicum , extracellular , nod factor , bradyrhizobium , biochemistry , calcium , chemistry , biology , microbiology and biotechnology , rhizobium , rhizobiaceae , symbiosis , bacteria , root nodule , enzyme , gene , genetics , organic chemistry
Summary Lipochito‐oligosaccharides (Nod factors) produced by Rhizobium or Bradyrhizobium are the key signal molecules for eliciting nodulation in their corresponding host legumes. To elucidate the signal transduction events mediated by Nod factors, we investigated the effects of Nod factors on the cytosolic [Ca 2+ ] of protoplasts prepared from roots and suspension‐cultured cells of soybean ( Glycine max and G. soja ) using a fluorescent Ca 2+ indicator, Fura‐PE3. NodBj‐V (C18:1, MeFuc), which is a major component of Nod factors produced by Bradyrhizobium japonicum , induces transient elevation of cytosolic [Ca 2+ ] in the cells of soybean within a few minutes. This effect is specific to soybean cells and was not observed in the tobacco BY‐2 cells. Furthermore, NodBj‐V without MeFuc did not induce any cytosolic [Ca 2+ ] elevation in soybean cells. Exclusion of Ca 2+ from the medium, as well as pre‐treatment of the cells with an external Ca 2+ chelator or with a plasma membrane voltage‐dependent Ca 2+ channel inhibitor, suppressed the Nod factor‐dependent cytosolic [Ca 2+ ] elevation. These results indicate that transient Ca 2+ influx from extracellular fluid is one of the earliest responses of soybean cells to NodBj‐V (C18:1, MeFuc) in a host‐specific manner.