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Arabidopsis ecotypes and mutants that are recalcitrant to Agrobacterium root transformation are susceptible to germ‐line transformation
Author(s) -
Mysore Kirankumar S.,
Kumar C. T. Ranjith,
Gelvin Stanton B.
Publication year - 2000
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2000.00646.x
Subject(s) - arabidopsis , transformation (genetics) , agrobacterium , biology , agrobacterium tumefaciens , mutant , arabidopsis thaliana , ecotype , microbiology and biotechnology , genetics , botany , gene
Summary Germ‐line transformation (vacuum infiltration) is frequently used to transform Arabidopsis thaliana using Agrobacterium tumefaciens . We have recently identified several Arabidopsis ecotypes and T‐DNA‐tagged mutants that are recalcitrant to Agrobacterium ‐mediated transformation of cut root segments. Some of these ecotypes and mutants are deficient in their ability to bind bacteria. Some are deficient in T‐DNA integration. We report here that using a germ‐line transformation protocol we transformed these ecotypes and mutants, including attachment‐ and integration‐defective Arabidopsis plants, with a frequency similar to that of highly susceptible wild‐type plants. However, we could not transform otherwise highly susceptible Arabidopsis plants by germ‐line or root transformation using several vir and attachment‐deficient Agrobacterium mutants. These results indicate that certain plant factors important for transformation may exist in germ‐line tissue but may be lacking in some somatic cells.