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Plant interstitial telomere motifs participate in the control of gene expression in root meristems
Author(s) -
Tremousaygue Dominique,
Manevski Alexandra,
Bardet Claude,
Lescure Nicole,
Lescure Bernard
Publication year - 1999
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1999.00627.x
Subject(s) - telomere , biology , arabidopsis , gene , genetics , promoter , saccharomyces cerevisiae , microbiology and biotechnology , conserved sequence , gene expression , computational biology , mutant , peptide sequence
Summary The promoters of Arabidopsis eEF1A genes contain a telomere motif, the telo ‐box, associated with an activating sequence, the tef ‐box. Database searches indicated the presence of telo‐ boxes in the 5′ region of numerous genes encoding components of the translational apparatus. By using several promoter constructs we demonstrate that the telo ‐box is required for the expression of a β‐glucoronidase gene in root primordia of transgenic Arabidopsis . This effect was observed when a telo ‐box was inserted upstream or downstream from the transcription initiation site, and occurred in synergy with the tef ‐box. These results clearly indicate that interstitial telomere motifs in plants are involved in control of gene expression. South‐western screening of a λZAP library with a double‐stranded Arabidopsis telomere motif resulted in characterization of a protein related to the conserved animal protein Purα. The possibility of a regulation process similar to that achieved by the Rap1p in Saccharomyces cerevisiae is discussed.