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Increased resistance to oxidative stress in transgenic tobacco plants overexpressing bacterial serine acetyltransferase
Author(s) -
Blaszczyk Anna,
Brodzik Robert,
Sirko Agnieszka
Publication year - 1999
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1999.00596.x
Subject(s) - glutathione , genetically modified crops , transgene , cysteine , oxidative stress , serine , transformation (genetics) , agrobacterium , biology , biochemistry , escherichia coli , acetyltransferase , nicotiana tabacum , gene , microbiology and biotechnology , chemistry , enzyme , acetylation
Summary Plant expression cassettes containing the Escherichia coli cysE gene alleles (encoding SAT) were constructed. After the Agrobacterium ‐mediated transformation of tobacco, we identified stable transformed plants containing several‐fold higher SAT activity in comparison to the control plant. Determination of non‐protein thiol contents indicated two‐ to threefold higher cysteine and glutathione levels in some of these transgenic plants. The maximal elevation of the cysteine level was about fourfold while that of GSH was about twofold higher than in the controls. The most striking physiological consequence of the modification of sulfur metabolite levels in the transgenic plants, however, was their several‐fold increased resistance to oxidative stress generated by exogenous hydrogen peroxide.