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Transcription activation mediated by the bZIP factor SPA on the endosperm box is modulated by ESBF‐1 in vitro
Author(s) -
Conlan R. Steven,
HammondKosack Michael,
Bevan Michael
Publication year - 1999
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1999.00522.x
Subject(s) - endosperm , transcription factor , promoter , biology , activating transcription factor , sp3 transcription factor , transcription (linguistics) , genetics , taf2 , microbiology and biotechnology , general transcription factor , gene , gene expression , linguistics , philosophy
Summary A modified in vitro transcription system has been used to study the function of the cloned bZIP transcription factor SPA and the binding activity ESBF I in activating transcription from the bifactorial endosperm box region of the wheat prolamin LMWG‐1D1 gene. Recombinant SPA expressed in Escherichia coli activated transcription from the endosperm box motif, and this was dependent upon the binding of the nuclear protein ESBF I. ESBF I did not activate transcription independently, but potentiated SPA‐mediated transcriptional activation. ESBF I is likely to be the equivalent of, or contain the recently characterised DOF class of, Zn‐finger protein called WPBF. These data provide new information about the interplay of members of the bZIP and DOF transcription factor families in regulating expression from bifactorial sites found in a variety of plant promoters.