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Stable chloroplast transformation in potato: use of green fluorescent protein as a plastid marker
Author(s) -
Sidorov Vladimir A.,
Kasten Daniel,
Pang ShengZhi,
Hajdukiewicz Peter T. J.,
Staub Jeffrey M.,
Nehra Narender S.
Publication year - 1999
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1999.00508.x
Subject(s) - plastid , transformation (genetics) , biology , green fluorescent protein , chloroplast , genome , botany , genetics , gene
Summary We describe here the development of a reproducible plastid transformation system for potato and regeneration of plants with uniformly transformed plastids. Two distinct tobacco‐specific plastid vectors, pZS197 (P rrn /a adA /T psbA ) and pMON30125 (P rrn / GFP/ T rps16:: P psbA/aadA /T psbA ), designed for integration into the large single copy and inverted repeat regions of the plastid genome, respectively, were bombarded into leaf explants of potato line FL1607. A total of three transgenic lines were selected out of 46 plates bombarded with pZS197 and three transgenic lines out of 104 plates were obtained with pMON30125. Development of a high frequency leaf‐based regenera‐ tion system, a stringent selection scheme and optimization of biolistic transformation protocol were critical for recovery of plastid transformants. Plastid‐expressed green fluorescent protein was used as a visual marker for identification of plastid transformants at the early stage of selection and shoot regeneration. The establishment of a plastid transformation system in potato, which has several advantages over routinely used nuclear transformation, offers new possibilities for genetic improvement of this crop.