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The cell cycle dependent phosphorylation of histone H3 is correlated with the condensation of plant mitotic chromosomes
Author(s) -
Houben Andreas,
Wako Toshiyuki,
FurushimaShimogawara Rieko,
Presting Gernot,
Künzel Gottfried,
Schubert Ingo,
Fukui Küchi
Publication year - 1999
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1999.00496.x
Subject(s) - biology , prophase , histone h3 , mitosis , telophase , premature chromosome condensation , chromatin , centromere , secale , histone , microbiology and biotechnology , dicentric chromosome , vicia faba , heterochromatin , chromosome , genetics , meiosis , botany , karyotype , dna , gene
Summary Mitotically dividing cells of Secale cereale , Hordeum vulgare and Vicia faba were studied by indirect immunofluorescence using an antibody recognizing phosphorylated histone H3. The study revealed the following features: (i) the H3 phosphorylation starts at prophase and ends at telophase in the pericentromeric chromatin, is associated with the condensation of mitotic chromosomes and is independent of the distribution of late replicating heterochromatin. (ii) Compared with other chromosome regions, the pericentromeric chromatin is histone H3 hyperphos‐ phorylated. (iii) The study of a semi‐dicentric chromo‐ some revealed that only at intact centromeres is the chromatin hyperphosphorylated at H3.