Premium
A signaling pathway, independent of the oxidative burst, that leads to hypersensitive cell death in cultured tobacco cells includes a serine protease
Author(s) -
Yano Akira,
Suzuki Kaoru,
Shinshi Hideaki
Publication year - 1999
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1999.00421.x
Subject(s) - respiratory burst , programmed cell death , reactive oxygen species , microbiology and biotechnology , proteases , signal transduction , serine protease , superoxide , oxidative phosphorylation , nadph oxidase , biology , biochemistry , chemistry , apoptosis , protease , enzyme
Summary To examine the role of reactive oxygen species (ROS) in the signal transduction that leads to hypersensitive cell death, we used a previously established system in which a xylanase from Trichoderma viride (TvX) induces an oxidative burst and cell death in a culture of tobacco cells. Diphenylene iodonium and N‐Acetyl‐L‐cysteine known as an inhibitor of NADPH oxidase and a scavenger of superoxides, respectively, and catalase inhibited the oxidative burst but did not inhibit the induction of cell death. We also found that inhibitors of serine proteases inhibited TvX‐induced cell death. These results suggest that there is a signaling pathway in which a serine protease might be responsible for the signal transduction, which is independent of the oxidative burst, that leads to the hypersensitive cell death of tobacco cells.