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Dissection of the ozone‐induced calcium signature
Author(s) -
Clayton Helen,
Knight Marc R.,
Knight Heather,
McAinsh Martin R.,
Hetherington Alistair M.
Publication year - 1999
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1999.00411.x
Subject(s) - ozone , glutathione , calcium , chemistry , fumigation , extracellular , cytosol , gene expression , intracellular , antioxidant , arabidopsis , glutathione s transferase , calcium signaling , biochemistry , microbiology and biotechnology , biophysics , gene , enzyme , biology , mutant , ecology , organic chemistry
Summary The cellular responses of plants to numerous extracellular stimuli are mediated by transient elevations in the concentration of cytosolic free calcium ([Ca 2+ ] cyt ). We have addressed the question of how cells can use this apparently ubiquitous system to initiate so many specific and appropriate end responses. We show that the pollutant gas ozone elicits a biphasic Ca 2+ response in intact Arabidopsis plants and a subsequent increase in expression of the gene encoding the antioxidant defence enzyme glutathione‐S‐transferase (GST). The second of the two [Ca 2+ ] cyt peaks, but not the first, could be eliminated either by pre‐treatment of plants with lanthanum chloride, or by reducing the duration of ozone fumigation. Under these conditions, ozone‐inducedGSTexpression was abolished. These data provide a functional dissection of the ozone Ca 2+ signalling pathway and indicate that the second ozone‐induced [Ca 2+ ] cyt peak provides the necessary information to direct expression ofGST.