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Potato StubSNF1 interacts with StubGAL83: a plant protein kinase complex with yeast and mammalian counterparts
Author(s) -
Lakatos Lóránt,
Klein Mathieu,
Höfgen Rainer,
Bánfalvi Zsófia
Publication year - 1999
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1999.00406.x
Subject(s) - yeast , protein kinase a , biology , microbiology and biotechnology , kinase , genetics
Summary StubSNF1 is a potato cDNA that encodes a protein kinase similar to the yeastSNF1gene involved in transcriptional regulation of glucose‐repressible genes. The yeast SNF1 functions in a complex with GAL83/SIP1/SIP2 and SNF4 proteins. We have used StubSNF1 as bait in a yeast two‐hybrid system to screen for potato cDNAs encoding proteins that bind to StubSNF1. Three overlapping cDNAs, two different in size, were isolated. DNA sequence analysis revealed that they were orthologues of the yeastGAL83/SIP1/SIP2genes and their mammalian counterparts,AMPK β‐subunits. The direct interaction between the potato proteins StubGAL83 and StubSNF1 was shown by anin vitrobinding assay. Southern and Northern hybridisations revealed thatStubGAL83exists in a low copy number in the potato genome and is highly (but organ‐specifically) expressed in potato. In contrast,StubSNF1possesses low transcript levels in each organ, except in flowers where high amounts of StubSNF1 mRNA could be detected. We demonstrate here that StubGAL83 can also interact with yeast SNF4 in a yeast two‐hybrid system suggesting that plant SNF1 kinases may function in complexes similar to those detected in yeast and mammals.