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Regulation of a putative high‐affinity nitrate transporter ( Nrt2;1At ) in roots of Arabidopsis thaliana
Author(s) -
Zhuo Degen,
Okamoto Mamoru,
Vidmar J. John,
Glass Anthony D. M.
Publication year - 1999
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1999.00396.x
Subject(s) - gene , transporter , biology , arabidopsis thaliana , nucleotide , amino acid , arabidopsis , microbiology and biotechnology , biochemistry , mutant
Summary Putative high‐affinity nitrate (NO 3 – ) transporter genes, designatedNrt2;1AtandNrt2;2At, were isolated fromArabidopsis thalianaby RT–PCR using degenerate primers. The genes shared 86% and 89% identity at the amino acid and nucleotide levels, respectively, while their proteins shared 30–73% identities with other eukaryotic highaffinity NO 3 – transporters. Both genes were induced by NO 3 – , butNrt2;1Atgene expression was not apparent in 2‐ and 5‐day‐old plants. By 10 days, and thereafter,Nrt2;1Atgene expression in roots was substantially higher than for theNrt2;2Atgene. RootNrt2;1Atexpression levels were strongly correlated with inducible high‐affinity 13 NO 3 – influx into intact roots under several treatment conditions. The use of inhibitors of N assimilation indicated that downregulation ofNrt2;1Atexpression was mediated by NH 4 + , gln and other amino acids.