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The self‐incompatibility ( S ) locus in Petunia hybrida is located on chromosome III in a region, syntenic for the Solanaceae
Author(s) -
ten Hoopen Rogier,
Harbord Robin M.,
Maes Tamara,
Nanninga Nanne,
Robbins Timothy P.
Publication year - 1998
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1998.00336.x
Subject(s) - locus (genetics) , biology , genetics , synteny , petunia , gene mapping , microbiology and biotechnology , gene , chromosome
Summary Seven independent transformants were previously shown to carry T‐DNA inserts that are genetically linked to the self‐incompatibility (S)‐locus inPetunia hybrida. These T‐DNAs provided reliable targets for fluorescentin situhybridisation (FISH) localisation. The T‐DNA loci were found to be distributed over the entire long arm of chromosome III. The most tightly linked T‐DNA loci were located in a subcentromeric position. Independent confirmation of this assignment of theS‐locus to chromosome III was obtained by RFLP analysis of a cDNA marker linked to theS‐locus of potato (CP100). Tight linkage was found between CP100 and a peroxidase isozyme locus previously mapped to chromosome III. The co‐localisation of CP100, peroxidase and theS‐locus in petunia reveals synteny around theS‐locus between four members of the Solanaceae family. The implications of a subcentromeric localisation of theS‐locus are discussed in relation to chromosome fragments associated with self‐compatible mutants.