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Retrotransposon‐based insertion polymorphisms (RBIP) for high throughput marker analysis
Author(s) -
Flavell Andrew J.,
Knox Maggie R.,
Pearce Stephen R.,
Ellis T. H. Noel
Publication year - 1998
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1998.00334.x
Subject(s) - transposable element , retrotransposon , biology , genetics , polymerase chain reaction , dna , computational biology , microbiology and biotechnology , genome , gene
Summary Two assays based upon PCR detection of a polymorphic PDR1 retrotransposon insertion inPisum sativumhave been developed. Both methods involve PCR with primers derived from the transposon and flanking DNA. The first method uses a dot assay for PCR product detection which could be fully automated for handling thousands of samples. The second method, which is designed to handle lower numbers, requires a single PCR and gel lane per sample. Both methods yield co‐dominant markers, with presence and absence of the transposon insertion independently scorable, and both could in principle be applied to any transposable element in any plant species.