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In situ monitoring of DNA: the plant nuclear envelope allows passage of short DNA fragments
Author(s) -
Gisel Andreas,
Rothen Barbara,
Iglesias Victor A.,
Potrykus Ingo,
Of Sautter Christ
Publication year - 1998
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1998.00323.x
Subject(s) - cytoplasm , dna , nucleus , nuclear dna , microbiology and biotechnology , in situ , cell nucleus , biology , envelope (radar) , chemistry , biophysics , genetics , mitochondrial dna , gene , telecommunications , radar , computer science , organic chemistry
Summary We monitored the cellular localization of fluorescently labeled foreign DNA in living plant cells. After physical delivery of labeled DNA fragments to the cytoplasm, short fragments up to 1.5 kb in length were found equally distributed between the cytoplasm and nucleus after 60 min. In contrast, 2.5 kb DNA fragments did not appear inside the nucleus. Thus, foreign DNA can enter plant nuclei through the intact nuclear envelope, but the efficiency of this process declines with increasing size of fragment.