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Reduced inositol content and altered morphology in transgenic potato plants inhibited for 1D‐ myo ‐inositol 3‐phosphate synthase
Author(s) -
Keller Ruth,
Brearley Charles A.,
Trethewey Richard N.,
MüllerRöber Bernd
Publication year - 1998
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1998.00309.x
Subject(s) - inositol , biochemistry , phosphate , solanum tuberosum , biology , raffinose , sucrose , inositol phosphate , chemistry , botany , receptor
SummaryMyo‐inositol is a precursor of many plant metabolites, including polyols, cell wall components and phosphoinositides. The first committed step in thede novo myo‐inositol synthetic pathway is catalysed by the enzyme 1D‐myo‐inositol 3‐phosphate synthase (MIPS;EC 5.5.1.4), which converts D‐glucose 6‐phosphate to 1D‐myo‐inositol 3‐phosphate. Suppression of MIPS activity by an antisense RNA approach in transgenic potato (Solanum tuberosumL.) plants to below 20% of the wild‐type level in leaves resulted in strongly reduced levels of inositol, galactinol and raffinose (approximately 7%, 5% and 12%, respectively, of wild‐type values). In contrast, increases were observed for concentrations of hexose phosphates (up to 1.7‐fold), sucrose (twofold) and starch (two‐ to fourfold). Transgenic plants exhibited reduced apical dominance, altered leaf morphology, precocious leaf senescence and a decrease in overall tuber yield. These observations indicate a crucial role formyo‐inositol in plant physiology and development.