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Mutations in Arabidopsis thaliana genes involved in the tryptophan biosynthesis pathway affect root waving on tilted agar surfaces
Author(s) -
Rutherford Robert,
Gallois Patrick,
Masson Patrick H.
Publication year - 1998
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1998.00279.x
Subject(s) - arabidopsis thaliana , mutant , biology , tryptophan , gene , phenotype , arabidopsis , biosynthesis , shoot , mutation , wild type , agar plate , botany , genetics , biochemistry , bacteria , amino acid
SummaryArabidopsis thalianaroots grow in a wavy pattern upon a slanted surface. A novel mutation in theanthranilate synthase α1(ASA1) gene, namedtrp5–2 wvc1, and mutations in thetryptophan synthase α and β1genes (trp3–1andtrp2–1, respectively) confer a compressed root wave phenotype on tilted agar surfaces. Whentrp5–2 wvc1seedlings are grown on media supplemented with anthranilate metabolites, their roots wave like wild type. Genetic and pharmacological experiments argue that the compressed root wave phenotypes oftrp5–2 wvc1,trp2–1andtrp3–1seedlings are not due to reduced IAA biosynthetic potential, but rather to a deficiency in L‐tryptophan (L‐Trp), or in a L‐Trp derivative. Although the roots of 7‐day‐old seedlings possess higher concentrations of free L‐Trp than the shoot as a whole,trp5–2 wvc1mutants show no detectable alteration in L‐Trp levels in either tissue type, suggesting that a very localized shortage of L‐Trp, or of a L‐Trp‐derived compound, is responsible for the observed phenotype.