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Control of expression of a gene encoding an extensin by phytochrome and a blue light receptor in spores of Adiantum capillus‐veneris L.
Author(s) -
Uchida Kenko,
Muramatsu Takamichi,
Jamet Elisabeth,
Furuya Masaki
Publication year - 1998
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1998.00264.x
Subject(s) - phytochrome , complementary dna , microbiology and biotechnology , biology , spore , gene expression , primer (cosmetics) , cdna library , gene , red light , botany , biochemistry , chemistry , organic chemistry
Summary In the present study, using a newly developed fluorescent differential display technique, we have carried out large‐scale screening for genes whose expression was regulated by phytochrome and antagonistically by a blue light receptor in the spores of the fernAdiantum capillus‐venerisL. Spores after imbibition were briefly irradiated with red, red/blue or blue light and collected 8 h after the irradiation. Total RNA was isolated from each sample and used to make cDNA with an oligo‐dT primer. The cDNA was then used as a template for PCR with the oligo‐dT primer and 80 arbitrary primers. The resulting PCR products were analyzed by an automated fluorescent DNA sequencer. Among 8000 displayed bands, we identified 15 upregulated and four down‐regulated bands by red light, and this red light effect was irreversibly reversed by blue light. We cloned one of the up‐regulated cDNA fragments and used it to screen a cDNA library prepared from the spores. The isolated insert is predicted to encode Ser‐(Pro)nrepeats and showed homology with cell wall‐associated extensins. The expression of this cDNA was induced 8 h after a red light treatment and the red light induction was photoreversibly prevented by far‐red light and photo‐irreversibly by blue light. The mRNA of this gene was detectable 4 h after red light irradiation and gradually increased in germinating spores.