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A gene encoding phosphatidylinositol‐4‐phosphate 5‐kinase is induced by water stress and abscisic acid in Arabidopsis thaliana
Author(s) -
Mikami Koji,
Katagiri Takeshi,
Iuchi Satoshi,
YamaguchiShinozaki Kazuko,
Shinozaki Kazuo
Publication year - 1998
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1998.00227.x
Subject(s) - arabidopsis , phosphatidylinositol , arabidopsis thaliana , abscisic acid , diacylglycerol kinase , second messenger system , biology , signal transduction , kinase , microbiology and biotechnology , biochemistry , gene , protein kinase c , mutant
Summary Phosphatidylinositol‐4‐phosphate 5‐kinase (PIP5K) phosphorylates phosphatidylinositol‐4‐phosphate to produce phosphatidylinositol‐4,5‐bisphosphate as a precursor of two second messengers, inositol‐1,4,5‐triphosphate and diacylglycerol, and as a regulator of many cellular proteins involved in signal transduction and cytoskeletal organization. Despite PIP5K playing such an essential role in a number of physiological processes, much still remains to be made clear about its association with plants. Searching theArabidopsisexpression sequence tag database against already known yeast and mammalian PIP5K cDNAs, we identified two clones which partly encode the sameArabidopsisPIP5K and isolated a corresponding full‐length cDNA encoding a protein that we designated AtPIP5K1. Recombinant AtPIP5K1 expressed inEscherichia colipossessed a PIP5K activityin vitro. Due to some structural and biochemical differences, AtPIP5K1 was not categorized as either a type I or type II PIP5K. The expression of the AtPIP5K1 mRNA was induced rapidly by treatingArabidopsisplants with drought, salt and abscisic acid, which suggests that AtPIP5K1 is involved in water‐stress signal transduction. These data give evidence for a close link between phosphoinositide signaling cascades and water‐stress responses in plants.