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The untranslated leader sequence of the barley lipoxygenase 1 ( Lox1 ) gene confers embryo‐specific expression
Author(s) -
Rouster Jacques,
Van Mechelen Jan,
CameronMills Verena
Publication year - 1998
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1998.00193.x
Subject(s) - embryo , lipoxygenase , biology , microbiology and biotechnology , sequence (biology) , gene , chemistry , biochemistry , enzyme
Summary The barleylipoxygenase 1(Lox1) gene encodes a protein expressed in embryos during grain development and germination and in leaves after methyl‐jasmonate (MeJA) treatment. Transient gene expression assays in germinating barley embryos were used to identifycis‐regulatory elements involved in the embryo‐specific expression of theLox1gene. Analysis of transcriptional or translational fusions betweenLox15′ upstream sequences and thegusAreporter gene indicated that the 5′‐untranslated leader sequence was involved in embryo‐specific expression. Replacement of the leader sequence from the aleurone‐specificChi26gene with theLox1leader sequence resulted in a chimeric gene expressed at high levels in embryo as well as in aleurone cells. Insertion of theLox1leader sequence between the 35S minimum promoter (A domain –90/+8) and thegusAreporter gene greatly enhanced promoter activity in a tissue‐specific manner. Deletion/replacement analysis of theLox1leader sequence, combined with transient expression in germinating embryos andin vitrotranscription/translation assays, suggests that theLox1leader sequence containscis‐elements regulating qualitative (tissue‐specific) and quantitative gene expression.