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A general method for gene isolation in tagging approaches: amplification of insertion mutagenised sites (AIMS)
Author(s) -
Frey Monika,
Stettner Cornelia,
Gierl Alfons
Publication year - 1998
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1998.00091.x
Subject(s) - transposable element , isolation (microbiology) , polymerase chain reaction , biology , gene , genetics , computational biology , dna , inverse polymerase chain reaction , bioinformatics , genome , nested polymerase chain reaction
A polymerase chain reaction (PCR) based procedure for the isolation of genes in transposon or T‐DNA tagging approaches has been developed. The method can be generally applied and allows the rapid isolation of putative gene sequences even in the presence of high numbers of insertion sequences. The technique has been used successfully for the isolation of the maize Bx1 gene tagged by a Mutator element.