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Lipochito‐oligosaccharides re‐initiate root hair tip growth in Vicia sativa with high calcium and spectrin‐like antigen at the tip
Author(s) -
D Norbert C. A.,
Rook Martin B.,
Bisseling Ton,
Emons Anne Mie C.
Publication year - 1998
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1998.00036.x
Subject(s) - root hair , tip growth , biophysics , cytoplasm , biology , microbiology and biotechnology , calcium , chemistry , botany , biochemistry , pollen tube , pollen , organic chemistry , pollination , gene
Summary Lipochito‐oligosaccharides, Nod factors secreted by Rhizobium bacteria, are signal molecules that induce deformation of root hairs of their host plant. A bioassay was used for deformation, and the cytological changes induced by specific lipochito‐oligosaccharides in root hairs of Vicia sativa L. (vetch), grown between glass slides, were examined. In the assay, root hairs of a particular developmental stage, those that were terminating growth, were susceptible to deformation. These hairs obtained characteristics of tip‐growing cells again: (i) a polar cytoplasmic organization and reverse fountain streaming, (ii) an accumulation of a spectrin‐like antigen at the tip, and (iii) a tip‐focused calcium gradient. Calcium gradients were visualized in Indo‐1 loaded root hairs with UV confocal microscopy and ratio‐imaging. The results show that hairs respond to the bacterial signal by recovering cytoplasmic polarity and exocytosis.