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Characterization of the targeted nuclear accumulation of GFP within the cells of transgenic plants
Author(s) -
Grebenok Robert,
Lambert Georgina,
Galbraith David
Publication year - 1997
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1997.d01-1.x
Subject(s) - nucleoplasm , biology , cytoplasm , microbiology and biotechnology , ribosome , nuclear localization sequence , green fluorescent protein , in vivo , cell nucleus , transgene , nuclear protein , in vitro , biochemistry , nucleolus , gene , genetics , rna , transcription factor
The soluble proteins of the nucleoplasm are synthesized on cytoplasmic ribosomes. Proteins larger than about 40 kDa are post‐translationally targeted to the nucleus via energy‐dependent processes, passing through the nuclear pore complex into the nucleoplasm. Targeting involves nuclear localization signals (NLSs) found within the primary sequences of the imported proteins. In higher plants, information has come primarily from study of proteins carrying ‘classical’ NLSs, comprising stretches of basic amino acids, and has required assays to measure nuclear uptake both in vitro and in vivo . In general, these assays are not entirely satisfactory; they are either technically demanding, are of limited accuracy and statistical rigor, or are unsuitable for in vivo applications.