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Map positions of 47 Arabidopsis sequences with sequence similarity to disease resistance genes
Author(s) -
Botella Miguel A.,
Coleman Mark J.,
Hughes Douglas E.,
Nishimura Marc T.,
Jones Jonathan D.G.,
Somerville Shauna C.
Publication year - 1997
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1997.12051197.x
Subject(s) - expressed sequence tag , biology , genetics , gene , arabidopsis , sequence tagged site , contig , restriction fragment length polymorphism , complementary dna , genome , gene mapping , bacterial artificial chromosome , chromosome , polymerase chain reaction , mutant
Summary Map positions have been determined for 42 non‐redundant Arabidopsis expressed sequence tags (ESTs) showing similarity to disease resistance genes (R‐ESTs), and for three Pto ‐like sequences that were amplified with degenerate primers. Employing a PCR‐based strategy, yeast artificial chromosome (YAC) clones containing the EST sequences were identified. Since many YACs have been mapped, the locations of the R‐ESTs could be inferred from the map positions of the YACs. R‐EST clones that exhibited ambiguous map positions were mapped as either cleavable amplifiable polymorphic sequence (CAPS) or restriction fragment length polymorphism (RFLP) markers using F 8 (Ler x Col‐0) recombinant inbred (RI) lines. In all cases but two, the R‐ESTs and Pto ‐like sequences mapped to single, unique locations. One R‐EST and one Pto ‐like sequence each mapped to two locations. Thus, a total of 47 loci were identified in this study. Several R‐ESTs occur in clusters suggesting that they may have arisen via gene duplication events. Interestingly, several R‐ESTs map to regions containing genetically defined disease resistance genes. Thus, this collection of mapped R‐ESTs may expedite the isolation of disease resistance genes. As the cDNA sequencing projects have identified an estimated 63% of Arabidopsis genes, a very large number of R‐ESTs (∼95), and by inference disease resistance genes of the leucine‐rich repeat‐class probably occur in the Arabidopsis genome.