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Existence of three regulatory regions each containing a highly conserved motif in the promoter of plastid‐encoded RNA polymerase gene ( rpoB )
Author(s) -
Inada Hitoshi,
Seki Motoaki,
Morikawa Hiromichi,
Nishimura Mitsuo,
Iba Koh
Publication year - 1997
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1997.11040883.x
Subject(s) - rpob , biology , rna polymerase , gene , plastid , genetics , microbiology and biotechnology , mutant , gene expression , rna , polymerase , chloroplast , 16s ribosomal rna
The plastid gene rpoB encodes a plastid‐specific, DNA‐dependent RNA polymerase. A study of a barley mutant, albostrians , indicated that rpoB itself is transcribed by a nuclear‐encoded RNA polymerase. However, the molecular nature of the nuclear‐encoded RNA polymerase and mechanisms of the transcriptional regulation of rpoB expression have not been elucidated. Using an in vivo transient assay system for gene expression in plastids, the 5′‐flanking region of rpoB from Arabidopsis thaliana in plastids of cultured BY‐2 tobacco cells was analyzed. A series of deletion analyses of the 5′‐flanking region indicated that there were two positive and one negative regulatory regions for rpoB expression. Comparison of the 5′‐flanking sequences of rpoB among several plant species revealed the existence of three conserved motifs in these regions.