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Cloning and expression of solanidine UDP‐glucose glucosyltransferase from potato
Author(s) -
Moehs Charles P.,
Allen Paul V.,
Friedman Mendel,
Belknap William R.
Publication year - 1997
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1997.11020227.x
Subject(s) - complementary dna , glucosyltransferase , biochemistry , messenger rna , biology , open reading frame , cdna library , microbiology and biotechnology , enzyme , yeast , peptide sequence , gene
A cDNA encoding solanidine glucosyltransferase (SGT) was isolated from potato. The cDNA was selected from a yeast expression library using a positive selection based on the higher toxicity of steroidal alkaloid aglycons relative to their associated glycosylated forms. The cDNA contained an open reading frame encoding a 56 kDa polypeptide with regions of similarity to previously characterized UDP‐glucosyltransferases. The enzyme activity and reaction products of recombinant SGT in yeast were consistent with those observed for the endogenous enzyme from potato. SGT mRNA and protein accumulated in tubers in response to wounding. The time course for SGT mRNA accumulation paralleled that of 3‐hydroxy‐3‐methylglutaryl‐coenzymeA isoform 1 ( hmg1 ) mRNA. Steady‐state SGT mRNA levels also increased transiently upon wounding of leaves.