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Construction of an ∼2 Mb contig in the region around 80 cM of Arabidopsis thaliana chromosome 2
Author(s) -
Wang Ming Li,
Huang Lu,
BongardPierce Deverie K.,
Belmonte Stephen,
Zachgo Eve Ann,
Morris John W.,
Dolan Maureen,
Goodman Howard M.
Publication year - 1997
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1997.00711.x
Subject(s) - contig , yeast artificial chromosome , arabidopsis thaliana , chromosome , genetics , bacterial artificial chromosome , biology , dna sequencing , gene , gene mapping , computational biology , genome , mutant
Summary A method for construction of bacterial artificial chromosome (BAC) contigs from a yeast artifical chromosome (YAC) physical map is described. An ∼2 Mb contig, consisting of two large BAC contigs linked by a small YAC, has been assembled in the region around 80 cM of Arabidopsis thaliana chromosome 2. Clones from this contig will facilitate gene isolation in the region and can be used directly as substrates for DNA sequencing.