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Requirement of sense transcription for homology‐dependent virus resistance and trans ‐inactivation
Author(s) -
English James J.,
Davenport Guy F.,
Elmayan Taline,
Vaucheret Hervé,
Baulcombe David C.
Publication year - 1997
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1997.00597.x
Subject(s) - transgene , biology , cauliflower mosaic virus , potato virus x , transcription (linguistics) , microbiology and biotechnology , tobacco etch virus , gene , genetically modified crops , promoter , virus , genetics , virology , plant virus , rna , gene expression , potyvirus , linguistics , philosophy
Summary Transgenic tobacco plants carrying post‐transcriptionally, silenced (L) or conditionally high‐expressing (H c ) cauliflower mosaic virus 35S promoter:β‐glucuronidase ( GUS ) transgenes were tested for their ability to support replication of a potato virus X derivative with an inserted GUS coding sequence (PVX.GUS). PVX.GUS accumulated to high levels on non‐transformed tobacco. In contrast, both L and H c tobacco were resistant to PVX.GUS. Another line, 271, carries a 35S‐driven transgene that can down‐regulate transcription from any other 35S promoter in trans . When the 271 locus was introduced into the L and H c lines, virus resistance was suppressed. The L tobacco line also trans ‐inactivated transient GUS expression from an extra‐chromosomal T‐DNA delivered by Agrobacterium . This trans ‐inactivation was not observed when the 271 locus was present. In addition, it was found that the 271 locus suppressed the virus resistance normally conferred by a 35S‐driven transgene derived from the PVX replicase open reading frame. These results indicate that sense transcription is required for a transgene to condition homology‐dependent virus resistance and post‐transcriptional trans ‐inactivation.