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A novel tetrasaccharide, with a structure similar to the terminal sequence of an arabinogalactan‐protein, accumulates in rice anthers in a stage‐specific manner
Author(s) -
Kawaguchi Kentaro,
Shibuya Naoto,
Ishii Tadashi
Publication year - 1996
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1996.9060777.x
Subject(s) - tetrasaccharide , oligosaccharide , arabinogalactan , chemistry , stamen , mass spectrometry , biochemistry , glycan , cell wall , polysaccharide , botany , biology , chromatography , glycoprotein , pollen
Summary Analysis of free sugars in developing rice anthers by high‐performance anion‐exchange chromatography (HPAEC) showed that a very high concentration of a novel oligosaccharide accumulated specifically during microsporogenesis. Structural analysis of the purified oligosaccharide by methylation analysis, mass spectrometry/mass spectrometry (MS/MS), and nuclear magnetic resonance (NMR) spectroscopy revealed its structure to be β‐ l Araf.(1→3)‐α‐ l ‐Ara f ‐(1→3)‐β‐ d ‐Gal p ‐(1→6)‐ d ‐Gal, which is closely related to a tetrasaccharide unit found in the glycan chain of a plant cell surface proteoglycan, the arabinogalactan‐protein (AGP). Chilling treatment (12°C, 4 days), which injures rice anthers during microsporogenesis, decreased the concentration of the tetrasaccharide, but the sucrose level increased. This effect was especially evident in a chilling‐sensitive mutant line, YM56‐1. These results suggest that this unique tetrasaccharide may play an important role in both the development of the rice anther and its response to chilling.