Premium
Detailed description of four YAC contigs representing 17 Mb of chromosome 4 of Arabidopsis thaliana ecotype Columbia
Author(s) -
Schmidt Renate,
West Joanne,
Cnops Gerda,
Love Karina,
Balestrazzi Alma,
Dean Caroline
Publication year - 1996
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1996.9050755.x
Subject(s) - contig , genetics , ecotype , biology , chromosome , gene mapping , cloning (programming) , positional cloning , genome , computational biology , gene , phenotype , computer science , programming language
Summary The detailed arrangement of 563 YAC clones comprising four contigs covering ∼17 Mbp of chromosome 4 is presented. YAC clones were positioned relative to each other and to markers by taking into account marker and end fragment hybridization data and the sizes of all YAC clones. This analysis made it possible to estimate physical distances between the majority of chromosome 4 markers. It also identified a relatively large number of YAC clones containing chimaeric inserts. The YAC contig map of the Columbia ecotype presents an important resource for map‐based cloning experiments, rapid mapping of DNA sequences and large‐scale genomic sequencing programs.