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A transcriptional activation domain of ATMYB2, a drought‐inducible Arabidopsis Myb‐related protein
Author(s) -
Urao Takeshi,
Noji Masaaki,
YamaguchiShinozaki Kazuko,
Shinozaki Kazuo
Publication year - 1996
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1996.10061145.x
Subject(s) - myb , arabidopsis , reporter gene , activator (genetics) , arabidopsis thaliana , gene , transcription factor , mutant , transcription (linguistics) , transcriptional regulation , n terminus , gatad2b , biology , microbiology and biotechnology , gene expression , biochemistry , peptide sequence , repressor , linguistics , philosophy
Summary Trans ‐activation activity of ATMYB2, a drought‐inducible Myb‐related protein in Arabidopsis thaliana , was analyzed using a transient assay of Arabidopsis leaf protoplasts. ATMYB2 activated the transcription of a reporter gene from the MYB‐binding site in a sequence‐specific manner. Deletion of the C‐terminal region of ATMYB2 reduced the trans ‐activation of the reporter gene, indicating that the acidic region at the C‐terminus of ATMYB2 is required for transcriptional activation. The domain exchange analysis with the yeast GAL4 revealed that the C‐terminal acidic region of ATMYB2 contains a sufficient domain for trans ‐activation. These results indicate that ATMYB2 acts as a transcriptional activator and that the C‐terminal acidic region of ATMYB2 can function as a transcriptional activation domain.