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Isolation and characterization of a cDNA encoding mitochondrial chaperonin 10 from Arabidopsis thaliana by functional complementation of an Escherichia coli groES mutant
Author(s) -
Koumoto Yasuko,
Tsugeki Ryuji,
Shimada Tomoo,
Mori Hitoshi,
Kondo Maki,
HaraNishimura Ikuko,
Nishimura Mikio
Publication year - 1996
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1996.10061119.x
Subject(s) - groes , biology , chaperonin , complementation , complementary dna , groel , microbiology and biotechnology , arabidopsis thaliana , mutant , biochemistry , escherichia coli , protein folding , gene
Summary Chaperonin (Cpn) is one of the molecular chaperones. Cpn10 is a co‐factor of Cpn60, which regulates Cpn60‐mediated protein folding. It is known that Cpn10 is located in mitochondria and chloroplasts in plant cells. The Escherichia coli homologue of Cpn10 is called GroES. A cDNA for the Cpn10 homologue was isolated from Arabidopsis thaliana by functional complementation of the E. coli groES mutant. The cDNA was 647 bp long and encoded a polypeptide of 98 amino acids. The deduced amino acid sequence showed approximately 50% identity to mammalian mitochondrial Cpn10s and 30% identity to GroES. A Northern blot analysis revealed that the mRNA for the Cpn10 homologue was expressed uniformly in various organs and was markedly induced by heat‐shock treatment. The Cpn10 homologue was constitutively expressed in transgenic tobaccos. Immunogold and immunoblot analyses following the subcellular fractionation of leaves from transgenic tobaccos revealed that the Cpn10 homologue was localized in mitochondria and accumulated at a high level in transgenic tobaccos.