Premium
Identification of a salicylic acid‐responsive element in the promoter of the tobacco pathogenesis‐related β‐1,3‐glucanase gene, PR‐2d
Author(s) -
Shah Jyoti,
Klessig Daniel F.
Publication year - 1996
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1996.10061089.x
Subject(s) - glucanase , salicylic acid , pathogenesis , gene , identification (biology) , genetics , promoter , biology , medicine , gene expression , immunology , botany
Summary The tobacco pathogenesis‐related PR‐2d gene encodes an acidic β‐1,3‐glucanase. Expression of the PR‐2d:uidA(GUS) chimeric gene is induced in leaves undergoing the hyper‐sensitive resistance response to tobacco mosaic virus and after treatment with salicylic acid (SA), a chemical believed to play an important role(s) in disease resistance. We have constructed transgenic tobacco plants which carry various segments of the PR‐2d promoter fused to a heterologous core 35S promoter driving the uidA(GUS) reporter gene. Their analysis indicates that sequences from −364 to −288 upstream of the PR‐2d transcription start site confer a high level of activation by SA (20‐fold). Mutations within this sequence, located between −339 and −333, depressed SA activation. This region is also required for the SA‐inducibility of a truncated PR‐2d:GUS chimeric gene. Contained within this region is a 25 bp element located between −348 and −324 which was specifically recognized by nuclear factors from tobacco leaves. No conclusive differences were observed in the ability of proteins in nuclear extracts from water‐treated versus SA‐treated plants to bind to this cis element in vitro .