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Isolation and mapping of a new set of 129 RFLP markers in Arabidopsis thaliana using recombinant inbred lines
Author(s) -
Liu YaoGuang,
Mitsukawa Norihiro,
Lister Clare,
Dean Caroline,
Whittier Robert F.
Publication year - 1996
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1996.10040733.x
Subject(s) - biology , genetics , arabidopsis thaliana , synteny , gene mapping , restriction fragment length polymorphism , genome , genetic marker , recombinant dna , gene , chromosome , genotype , mutant
A new collection of 129 Arabidopsis thaliana RFLP markers has been established based upon DNA fragments cloned in the pUC119 plasmid vector and insert end sequences of P1 clones. Dominant/null alleles affecting low‐copy number sequences account for nine of the mapped polymorphisms, suggesting that deletions are not rare in A. thaliana . Recombinant inbred (RI) lines were used for mapping these marker loci. RI line‐based mapping allows integration of this set of markers with markers previously reported as well as with any markers mapped in the future using this replenishable mapping resource. These markers are useful for map‐based gene isolation and genome physical mapping in A. thaliana as well as studies of chromosome colinearity (synteny) with related species.