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Characterization and mapping of Ds —GUS‐T‐DNA lines for targeted insertional mutagenesis
Author(s) -
Smith D.,
Yanai Y.,
Liu Y.G.,
Ishiguro S.,
Okada K.,
Shibata D.,
Whittier R.F.,
Fedoroff N.V.
Publication year - 1996
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1996.10040721.x
Subject(s) - insertional mutagenesis , transposase , transposable element , transposition (logic) , genetics , biology , p element , transposon mutagenesis , mutagenesis , sleeping beauty transposon system , dna , mutant , microbiology and biotechnology , gene , computer science , artificial intelligence
The transposition patterns of the Ds —GUS transposon T‐DNA in 23 independent single‐copy lines have been characterized and the map positions of 10 of them on three of the five Arabidopsis chromosomes are reported. Using overexpressed Activator ( Ac ) elements as a transposase source, it was found that the primary determinant of transposition frequency is the insertion site of the Ac ‐T‐DNA. Neither the structure of the transposon T‐DNA nor, in most cases, its insertion site have a significant effect on transposition frequency. Both the frequency and timing of transposition are influenced by the parent through which the transposon and transposase T‐DNAs are transmitted. Overall, nearly 75% of plants in which excision has occurred bear a reinserted element and very short‐range transpositions predominate, underlining the advantage of using mapped transposons for insertional mutagenesis.