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Isolation and characterization of abscisic acid‐deficient Arabidopsis mutants at two new loci
Author(s) -
LéonKloosterziel Karen M.,
Gil Marta Alvarez,
Ruijs Gerda J.,
Jacobsen Steven E.,
Olszewski Neil E.,
Schwartz Steven H.,
Zeevaart Jan A.D.,
Koornneef Maarten
Publication year - 1996
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1996.10040655.x
Subject(s) - abscisic acid , mutant , arabidopsis , paclobutrazol , biology , gibberellin , phenotype , mutation , genetics , seed dormancy , dormancy , gene , germination , botany
Novel Arabidopsis mutants with lowered levels of endogenous abscisic acid (ABA) were isolated. These were selected in a screen for germination in the presence of the gibberellin biosynthesis inhibitor paclobutrazol. Another mutant was isolated in a screen for NaCl tolerance. The ABA‐deficiency was caused by two monogenic, recessive mutations, aba2 and aba3 , that were both located on chromosome 1. The mutants showed a phenotype that is known to be characteristic for ABA‐deficiency: a reduced seed dormancy and excessive water loss, leading to a wilty phenotype. Double mutant analysis, combining different aba mutations, indicated the leaky nature of the mutations.