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An S ‐locus‐related gene in Arabidopsis encodes a functional kinase and produces two classes of transcripts
Author(s) -
Tobias Christian M.,
Nasrallah June B.
Publication year - 1996
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1996.10030523.x
Subject(s) - arabidopsis , genetics , gene , locus (genetics) , biology , computational biology , mutant
ARK1 is a vegetatively expressed receptor protein kinase gene isolated from Arabidopsis thaliana based on its sequence similarity to Brassica genes involved in pollen‐stigma signaling and the self‐incompatibility response. This paper shows that the kinase domain of ARK1 auto‐phosphorylates on serine and threonine residues when expressed as a recombinant fusion protein. ARK1 produces a 2.9 kb transcript encoding a transmembrane receptor protein kinase and a 1.4 kb transcript encoding the receptor domain alone. Constitutive high‐level expression of ARK1 transcripts in transgenic Arabidopsis resulted in severe stunting and also disrupted normal cellular expansion and differentiation.