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rbohA , a rice homologue of the mammalian gp91phox respiratory burst oxidase gene
Author(s) -
Groom Quentin J.,
Torres Miguel Angel,
FordhamSkelton Anthony P.,
HammondKosack Kim E.,
Robinson Nigel J.,
Jones Jonathan D.G.
Publication year - 1996
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1996.10030515.x
Subject(s) - respiratory burst , gene , biology , complementary dna , superoxide , nadph oxidase , protein subunit , genomic dna , dna , oxidase test , genetics , biochemistry , microbiology and biotechnology , enzyme
It has been hypothesized that plants contain respiratory burst oxidases which, upon activation, oxidize NADPH and generate extracellular superoxide, O 2° − . These proteins are proposed to play a central role in defence against pathogens. However, plant DNA sequences that encode proteins with similarity to components of respiratory burst oxidases have not previously been reported. This paper describes the complete cDNA and genomic DNA sequence of the rice rbohA (for respiratory burst oxidase homologue) gene. The predicted RbohA product is most similar to the main catalytic subunit, gp91phox, of the respiratory burst oxidase of neutrophils. Reverse transcriptase PCR detects rbohA transcripts in both roots and shoots of healthy rice plants.