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The CER3 gene of Arabidopsis thaliana is expressed in leaves, stems, roots, flowers and apical meristems
Author(s) -
Hannoufa Abdelali,
Negruk Valentin,
Eisner Galina,
Lemieux Bertrand
Publication year - 1996
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1996.10030459.x
Subject(s) - biology , gene , mutant , genetics , complementary dna , meristem , complementation , locus (genetics) , microbiology and biotechnology , southern blot , arabidopsis thaliana , open reading frame , exon , start codon , arabidopsis , homology (biology) , nucleic acid sequence , northern blot , sequence analysis , peptide sequence , messenger rna
The eceriferum3 ( cer3 ) locus encodes one of 21 gene products known to be involved in wax biosynthesis in Arabidopsis thaliana . Mutations at these loci are readily identified by their bright, dark‐green stems when compared with the more glaucous wild‐type plant. Clones of a gene which encodes a 795 amino acid open reading frame have been isolated by using plant DNA flanking the site of a T‐DNA insertion in line BRL1. Molecular complementation of the cer3 mutant phenotype by clones of this gene establish that it corresponds to the CER3 gene. Although the 90 kDa predicted amino acid sequence of this gene shows no homology to any other known protein, the second exon of CER3 encodes a RRX 12 KK nuclear localization sequence (NLS). Southern blot and DNA sequence analysis revealed that the T‐DNA is inserted 89 bp downstream of the translation termination codon of this gene. Northern blot hybridization of RNA isolated from the BRL1 mutant with the CER3 cDNA probe indicated that the transcript is absent in this mutant line. Unlike other CER genes that have been cloned to date, high levels of the CER3 transcript were found in all tissues from wild‐type plants, that is, leaves, stems, roots, flowers, and apical meristems.