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A rolB regulatory factor belongs to a new class of single zinc finger plant proteins
Author(s) -
Paolis Angelo,
Sabatini Sabrina,
Pascalis Luca,
Costantino Paolo,
Capone Imerio
Publication year - 1996
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1996.10020215.x
Subject(s) - zinc finger , class (philosophy) , factor (programming language) , computational biology , zinc , biology , computer science , transcription factor , genetics , materials science , artificial intelligence , gene , programming language , metallurgy
A protein which binds to the regulatory domain B necessary for expression of the plant oncogene rolB in (root) meristems contains a single zinc finger of a novel type conserved in dicots and monocots. Band shift analysis revealed the presence in tobacco nuclei of a protein selectively binding to domain B, a tetramer of which was used to isolate a cDNA ( NtBBF1, N icotiana t abacum rol B domain B,F actor 1 ) from a tobacco expression library. The corresponding genomic clone was also isolated. The protein encoded by NtBBF1 contains a single C 2 C 2 zinc finger, and its target sequence in domain B was identified by means of mutagenized oligonucleotides. The DNA‐binding capability of the zinc finger was assessed by means of a fusion of this latter with the glutathione‐S‐transferase protein, that was shown to bind the same target sequence as NtBBF1. A number of other tobacco cDNAs encoding different proteins with a domain (BBF domain) encompassing the zinc finger identical to NtBBF1 were also isolated. Furthermore, a cDNA encoding a protein with an almost identical single zinc finger was isolated from Arabidopsis . A very closely related zinc finger has very recently been identified in maize transcription factors and termed the Dof domain. It is proposed that the tobacco, Arabidopsis and maize BBF/Dof domain proteins are members of a new broad family of plant transcription factors acting through a single zinc finger widely utilized in the plant kingdom.