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A protocol for obtaining embryogenic cell lines from Arabidopsis
Author(s) -
Pillon Elisa,
Terzi Mario,
Baldan Barbara,
Mariani Paola,
Schiavo Fiorella Lo
Publication year - 1996
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1996.09040573.x
Subject(s) - somatic embryogenesis , arabidopsis , zygote , biology , somatic cell , arabidopsis thaliana , embryo , auxin , microbiology and biotechnology , botany , ecotype , genetics , embryogenesis , gene , mutant
Embryogenic cell lines with lasting embryogenic potential can be obtained from somatic embryos induced directly from zygotic embryos of Arabidopsis thaliana , ecotype Columbia. The response to a critical concentration of auxin, which seems to be the all‐important factor in the generation of embryogenic cell lines, is exhibited by somatic embryos but not by zygotic ones. The basis for this differential response remains obscure and will be discussed in relation to other systems.