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Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non‐redundant ESTs
Author(s) -
Cooke Richard,
Raynal Monique,
Laudié Michele,
Grellet Françoise,
Delseny Michel,
Morris PeterChristian,
Guerrier Danièle,
Giraudat Jérôme,
Quigley Françoise,
Clabault Gérard,
Li YouFang,
Mache Régis,
Krivitzky Micheline,
Gy Isabelle JeanJacques,
Kreis Martin,
Lecharny Alain,
Parmentier Yves,
Marbach Jacqueline,
Fleck Jacqueline,
Clément Bernadette,
Philipps Gabriel,
Hervé Christine,
Bardet Claude,
Tremousaygue Dominique,
Lescure Bernard,
Lacomme Christophe,
Roby Dominique,
Jourjon MarieFrançoise,
Chabrier Patrick,
Charpenteau JeanLouis,
Desprez Thierry,
Amselem Joelle,
Chiapello Helen,
Höfte Herman
Publication year - 1996
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1996.09010101.x
Subject(s) - expressed sequence tag , gene , biology , genome , genetics , complementary dna , arabidopsis , computational biology , arabidopsis thaliana , cdna library , mutant
Nearly 7000 Arabidopsis thaliana ‐expressed sequence tags (ESTs) from 10 cDNA libraries have been sequenced, of which almost 5000 non‐redundant tags have been submitted to the EMBL data bank. The quality of the cDNA libraries used is analysed. Similarity searches in international protein data banks have allowed the detection of significant similarities to a wide range of proteins from many organisms. Alignment with ESTs from the rice systematic sequencing project has allowed the detection of amino acid motifs which are conserved between the two organisms, thus identifying tags to genes encoding highly conserved proteins. These genes are candidates for a common framework in genome mapping projects in different plants.