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In vitro transcription of plant RNA polymerase I‐dependent rRNA genes is species‐specific
Author(s) -
Fan Hao,
Yakura Kimitaka,
Miyanishi Masako,
Sugita Mamoru,
Sugiura Masahiro
Publication year - 1995
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1995.08020295.x
Subject(s) - biology , rna polymerase i , rna polymerase ii , transcription (linguistics) , transcription factor ii d , ribosomal rna , rna polymerase , microbiology and biotechnology , polymerase , gene , nicotiana tabacum , transcription factories , genetics , rna , gene expression , promoter , linguistics , philosophy
An in vitro transcription system derived from tobacco ( Nicotiana tabacum ) cultured cell (BY‐2) nuclei supports transcription of the RNA polymerase I‐dependent tobacco rRNA gene. The transcription initiation site determined in vitro was found at residue A which corresponds to that lying within the consensus sequence surrounding plant pre‐rRNA initiation sites. The tobacco rRNA gene is actively transcribed in the tobacco nuclear extract while the broad bean rRNA gene is inactive in the heterologous system, indicating that plant RNA polymerase I‐dependent transcription is species‐specific.