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Molecular cloning of cDNAs encoding the protein backbones of arabinogalactan‐proteins from the filtrate of suspension‐cultured cells of Pyrus communis and Nicotiana alata
Author(s) -
Mau ShaioLim,
Chen ChaoGuang,
Pu ZhaoYan,
Moritz Robert L.,
Simpson Richard J.,
Bacic Antony,
Clarke Adrienne E.
Publication year - 1995
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1995.08020269.x
Subject(s) - arabinogalactan , complementary dna , amino acid , peptide sequence , biochemistry , biology , gene , microbiology and biotechnology , cell wall
This paper reports the isolation of cDNAs encoding the protein backbone of two arabinogalactan‐proteins (AGPs), one from pear cell suspension cultures (AGP Pc 2) and the other from suspension cultures of Nicotiana alata (AGP Na 2). The proteins encoded by these cDNAs are quite different from the ‘classical’ AGP backbones described previously for AGPs isolated from pear suspension cultures and extracts of N. alata styles. The cDNA for AGP Pc 2 encodes a 294 amino acid protein, of which a relatively short stretch (35 amino acids) is Hyp/Pro rich; this stretch is flanked by sequences which are dominated by Asn residues. Asn residues are not a feature of the ‘classical’ AGP backbones in which Hyp/Pro, Ser, Ala and Thr account for most of the amino acids. The cDNA for AGP Na 2 encodes a 437 amino acid protein, which contains two distinct domains: one rich in Hyp/Pro, Ser, Ala, Thr and the other rich in Asn, Tyr and Ser. The composition and sequence of the Pro‐rich domain resembles that of the ‘classical’ AGP backbone. The Asn‐rich domains of the two cDNAs described have no sequence similarity; in both cases they are predicted to be processed to give a mature backbone with a composition similar to that of the ‘classical’ AGPs. The study shows that different AGPs can differ in the amino acid sequence in the protein backbone, as well as the composition and sequence of the arabinogalactan side‐chains. It also shows that differential expression of genes encoding AGP protein backbones, as well as differential glycosylation, can contribute to the tissue specificity of AGPs.