Premium
Germin‐like oxalate oxidase, a H 2 O 2 ‐producing enzyme, accumulates in barley attacked by the powdery mildew fungus
Author(s) -
Zhang Ziguo,
Collinge David B.,
ThordalChristensen Hans
Publication year - 1995
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1995.08010139.x
Subject(s) - powdery mildew , hordeum vulgare , oxalate , inoculation , oxidase test , enzyme , fungus , mildew , enzyme assay , biology , chemistry , biochemistry , botany , horticulture , poaceae , inorganic chemistry
Germin‐like oxalate oxidase is an oligomeric enzyme which generates H 2 O 2 . This paper reports increased activity of this enzyme in association with the response of barley to the powdery mildew fungus, Erysiphe graminis f.sp. hordei . The increase is detected in a colorimetric assay as well as on activity blots using extracts of both resistant and susceptible leaves. The increase is generally apparent from 24 h after inoculation. From 48 h after inoculation, there is an approximately 10‐fold higher oxalate oxidase activity in the samples from inoculated plants compared with the controls. The oxalate oxidase activity increase appears 1–3 days earlier than PR‐1 protein accumulation. SDS—PAGE analysis suggests that this pathogen‐response oxalate oxidase is different from a commercially available barley oxalate oxidase, and from a constitutive barley root oxalate oxidase.