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Early nodulin gene expression during Nod factor‐induced processes in Vicia sativa
Author(s) -
Vijn Irma,
MartinezAbarca Francisco,
Yang WeiCai,
Neves Lucinda,
Brussel Anton,
Kammen Ab,
Bisseling Ton
Publication year - 1995
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1995.08010111.x
Subject(s) - nod factor , primordium , nod , biology , microbiology and biotechnology , rhizobium leguminosarum , root hair , nodule (geology) , gene expression , vicia faba , rhizobium , botany , gene , root nodule , rhizobiaceae , genetics , bacteria , symbiosis , paleontology
Rhizobium leguminosarum bv. viciae ‐secreted Nod factors are able to induce root hair deformation, the formation of nodule primordia and the expression of early nodulin genes in Vicia sativa (vetch). To obtain more insight into the mode of action of Nod factors the expression of early nodulin genes was followed during Nod factor‐induced root hair deformation and nodule primordium formation. The results of these studies suggested that the expression of VsENOD5 and VsENOD12 is not required for root hair deformation. In the Nod factor‐induced primordia both VsENOD12 and VsENOD40 are expressed in a spatially controlled manner similar to that found in Rhizobium ‐induced nodule primordia. In contrast, VsENOD5 expression has never been observed in Nod factor‐induced primordia, showing that the induction of VsENOD5 and VsENOD12 expression are not coupled. VsENOD5 expression is induced in the root epidermis by Nod factors and in Rhizobium ‐induced nodule primordia only in cells infected by the bacteria, suggesting that the Nod factor does not reach the inner cortical cells.