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Functional dissection of the promoter of the pollen‐specific gene NTP303 reveals a novel pollen‐specific, and conserved cis ‐regulatory element
Author(s) -
Weterings Koen,
Schrauwen Jan,
Wullems George,
Twell David
Publication year - 1995
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1995.08010055.x
Subject(s) - gene , regulatory sequence , promoter , biology , genetics , pollen , conserved sequence , regulation of gene expression , microbiology and biotechnology , gene expression , peptide sequence , botany
Regulatory elements within the promoter of the pollen‐specific NTP303 gene from tobacco were analysed by transient and stable expression analyses. Analysis of precisely targeted mutations showed that the NTP303 promoter is not regulated by any of the previously described pollen‐specific cis ‐regulatory elements. However, two adjacent regions from −103 to −86 bp and from −86 to −59 bp were shown to contain sequences which positively regulated the NTP303 promoter. Both of these regions were capable of driving pollen‐specific expression from a heterologous promoter, independent of orientation and in an additive manner. The boundaries of the minimal, functional NTP303 promoter were determined to lie within the region −86 to −51 bp. The sequence AAATGA localized from −94 to −89 bp was identified as a novel cis ‐acting element, of which the TGA triplet was shown to comprise an active part. This element was shown to be completely conserved in the similarly regulated promoter of the Bp10 gene from Brassica napus encoding a homologue of the NTP303 gene.