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Somatic and germinal activities of maize Activator ( Ac ) transposase mutants in transgenic tobacco
Author(s) -
Kunze Reinhard,
Kühn Sandra,
Jones Jonathan D.G.,
Scofield Steven R.
Publication year - 1995
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1995.08010045.x
Subject(s) - transposase , transposable element , mutant , biology , wild type , cauliflower mosaic virus , genetics , transgene , gene , genetically modified crops
The properties have been investigated of two deletion derivatives of the transposase protein (TPase) of maize transposable element Ac in transgenic tobacco. The wild‐type and mutant TPases were expressed as fusions to the cauliflower mosaic virus 35S promoter. A deletion of 102 amino acids from the N‐terminus, TPase(103–807), induces Ds excisions from a SPT::Ds reporter locus with a higher frequency than the wild‐type TPase. The increased transpositional activity of TPase(103–807) is a dominant trait, as seedlings coexpressing truncated and wild‐type TPase show the characteristic TPase(103–807) variegation pheno‐type. A transpositionally inactive TPase deletion derivative lacking 188 amino acids from the N‐terminus inhibits the transpositional activity of the wild‐type TPase.