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Correlation between the induction of a gene for Δ 1 ‐pyrroline‐5‐carboxylate synthetase and the accumulation of proline in Arabidopsis thaliana under osmotic stress
Author(s) -
Yoshiba Yoshu,
Kiyosue Tomohiro,
Katagiri Takeshi,
Ueda Hiroko,
Mizoguchi Tsuyoshi,
YamaguchiShinozaki Kazuko,
Wada Keishiro,
Harada Yoshinori,
Shinozaki Kazuo
Publication year - 1995
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1995.07050751.x
Subject(s) - arabidopsis thaliana , proline , biology , complementary dna , biochemistry , amino acid , cdna library , microbiology and biotechnology , gene , mutant
The isolation and characterization is reported of a cDNA for Δ 1 ‐pyrroline‐5‐carboxylate (P5C) synthetase (cAtP5CS), an enzyme involved in the biosynthesis of proline, from a cDNA library prepared from a dehydrated rosette plant of Arabidopsis thaliana . Southern blot analysis suggested that only one copy of the corresponding gene ( AtP5CS ) is present in A. thaliana . The deduced amino acid sequence of the P5CS protein (AtP5CS) from A. thaliana exhibited 74% homology to that of the P5CS from Vigna aconitifolia . Northern blot analysis revealed that the gene for P5CS was induced by dehydration, high salt and treatment with ABA, while it was not induced by heat or cold treatment. Moreover, the simultaneous accumulation of proline was observed as a result of the former treatments in A. thaliana . A cDNA for P5C reductase (cAtP5CR) was also isolated from A. thaliana and Northern blot analysis was performed. The AtP5CR gene was not induced to a significant extent by dehydration or high‐salt stress. These observations suggest that the AtP5CS gene plays a principal role in the biosynthesis of proline in A. thaliana under osmotic stress.