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Production of fertile transgenic maize plants by silicon carbide whisker‐mediated transformation
Author(s) -
Frame Bronwyn R.,
Drayton Paul R.,
Bagnall Susan V.,
Lewnau Carol J.,
Bullock W. Paul,
Wilson H. Martin,
Dunwell James M.,
Thompson John A.,
Wang Kan
Publication year - 1994
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1994.6060941.x
Subject(s) - callus , transformation (genetics) , biology , transgene , genetically modified rice , genetically modified crops , gene , genetically modified maize , botany , genetics
Summary A simple and inexpensive system for the generation of fertile, transgenic maize plants has been developed. Cells from embryogenic maize suspension cultures were transformed using silicon carbide whiskers to deliver plasmid DNA carrying the bacterial bar and uidA (gus) genes. Transformed cells were selected on medium containing the herbicide bialaphos. Integration of the bar gene and activity of the enzyme phosphinothricin acetyl transferase (PAT) were confirmed in all bialaphos‐resistant callus lines analysed. Fertile transgenic maize plants were regenerated. Herbicide spraying of progeny plants revealed that the bar gene was transmitted in a Mendelian fashion.